Daniel von Wangenheim

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Curriculum vitae: 
since 2015Postdoc, Jiri Friml Group 
Developmental and Cell Biology of Plants
Institute of Science and Technology Austria (IST Austria)
2010-2015Ph. D. student, Ernst Stelzer Group
Buchmann Institute for Molecular Life Sciences
Johann-Wolfgang-Goethe-Universität Frankfurt am Main (Goethe University Frankfurt am Main, Germany)
Preliminary title of the thesis: “4D-imaging of Arabidopsis thaliana root development in close-to natural growth condition using light-sheet based fluorescent microscopy”
2009Diploma, Biology
Institute for Cellular and Molecular Botany
Rheinische Friedrich-Wilhelms Universität Bonn (University of Bonn, Germany)
Titel of the thesis: “Visualisierung dynamischer vesikulärer Kompartimente in Wurzelhaarzellen von Arabidopsis thaliana”
2002–2009Student, Biology
Johann-Wolfgang-Goethe-Universität Frankfurt am Main (Goethe University Frankfurt am Main, Germany)
and Rheinische Friedrich-Wilhelms Universität Bonn (University of Bonn, Germany).



Plant imaging at the cellular level with high spatial and temporal resolution requires non-invasive and viable imaging methods. We suggest Light Sheet-based Fluorescence Microscopy (LSFM) to study the dynamics of plant growth at organ, cellular and sub-cellular levels over periods of time, ranging from seconds to days.  During the recording process the plant continues to grow in an upright position with its leaves in the air and its root in the medium while a perfusion system provides fresh medium.  The leaves are illuminated with a standard laboratory light source in order to ensure physiological growth conditions (Maizel & von Wangenheim 2011). With LSFM we are able to quantify the development of lateral root primordia and the diurnal growth rhythm of Arabidopsis thaliana lateral roots.  We apply LSFM at higher spatial and temporal resolution by recording cell divisions and movements of single endosomes in live growing root samples. 

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* equal contributors

von Wangenheim D., Fangerau J., Schmitz A., Smith R. S., Leitte H., Stelzer E. H. K., & Maizel A. (2016). Rules and Self-Organizing Properties of Post-embryonic Plant Organ Cell Division Patterns. Current Biology. doi:10.1016/j.cub.2015.12.047

von Wangenheim D., Rosero A., Komis G., Šamajová O., Ovečka M., Voigt B., & Šamaj J. (2016). Endosomal Interactions during Root Hair Growth. Frontiers in Plant Science, 6. doi:10.3389/fpls.2015.01262

Berson, T.*, von Wangenheim D.*, Takác T., Šamajová O., Rosero A., Ovecka M., Komis G., Stelzer E.H.K, and Šamaj, J. (2014). Trans-golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth. BMC Plant Biology, 14(1):252.

Vermeer J. E. M., von Wangenheim D., Barberon M., Lee Y., Stelze, E.H.K., Maizel A., and Geldner N. (2014). A spatial accommodation by neighboring cells is required for organ initiation in Arabidopsis. Science, 343(6167):178–183.PMID: 24408432.

von Wangenheim, D., Daum G., Lohmann J.U., Stelzer E.H.K., and Maizel A., 2014. Live Imaging of Arabidopsis Development. In Arabidopsis Protocols, edited by Jose J. Sanchez-Serrano and Julio Salinas, 539–550. Methods in Molecular Biology 1062. Humana Press. http://link.springer.com/protocol/10.1007/978-1-62703-580-4_28.

Rosquete, M.R., von Wangenheim D., Marhavý P., Barbez E., Stelzer E.H.K., Benková E., Maizel A., and Kleine-Vehn J. (2013) An Auxin Transport Mechanism Restricts Positive Orthogravitropism in Lateral Roots. Current Biology. doi:10.1016/j.cub.2013.03.064.

Lucas, M., Kenobi, K., von Wangenheim D., Voß U., Swarup K., Smet I. D., Damme D. V., Lawrence T., Péret B., Moscardi E., Barbeau D., Godin C., Salt D., Guyomarch S., Stelzer E. H. K., Maizel A., Laplaze L., and Bennett M. J. (2013). Lateral root morphogenesis is dependent on the mechanical properties of the overlaying tissues. Proceedings of the National Academy of Sciences, doi: 10.1073/pnas.1210807110

Maizel A*, von Wangenheim D.*, Federici F, Haseloff J, Stelzer E.H.K (2011). High resolution, live imaging of plant growth in near physiological bright conditions using light sheet fluorescence microscopy. The Plant Journal, 68 (2): 377-385

Šamajová O, Takáč T, Wangenheim D. von, Stelzer E.H.K, Šamaj J (2012). Update on methods and techniques to study endocytosis in plants. In Šamaj, J., editor, Endocytosis in Plants, pages 1–36. Springer Berlin Heidelberg

Chen T, Wang X, von Wangenheim D., Zheng M, Šamaj J, Ji W, and Lin J (2012). Probing and tracking organelles in living plant cells. Protoplasma, 249(2):157–167