From the Ultramikroskop to 4Pi Microscopy to Theta Microscopy to Tetrahedral Microscopy to Light sheet-based fluorescence microscopy (LSFM)

Specimens scatter and absorb light, thus, the delivery of the probing light and the collection of the signal light become inefficient; many endogenous biochemical compounds also absorb light and suffer degradation of some sort (photo-toxicity), which induces malfunction of a specimen.  In conventional and confocal fluorescence microscopy, whenever a single plane is observed, the entire specimen is illuminated (Verveer 2007).  Recording stacks of images along the optical z-axis thus illuminates the entire specimen once for each plane.  Hence, cells are illuminated 10-20 and fi

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